Abstract：The effects of taurine(Tau) on expressions of GRP78,Caspase-12 of the renal tissue at rats in renal ischemia /reperfusion(I/R) injury was observed.Thirty Wistar rats of either sex were randomly divided into sham operation group(control),I/R group and I/R+Tau group.The animal model of acute renal I/R injury was prepared by clamping bilateral renal pedicle for 45 mins and then reperfusion over 24 hours.I/R+Tau group was treated with intraperitoneal injection of 200 mg·kg-1 Tau solution 1 hour before surgery,and the rest operations followed the same as that in I/R group.The blood urea nitrogen(BUN) and creatinine(Cr) were detected.The histological changes of renal were observed by HE staining;the mRNA and protein expressions of GRP78.Caspase-12 in the renal tissue were analyzed by RT-PCR and immunohistochemistry,respectively.Compared with the Sham group,the serum BUN,Cr in I/R group had significant increases,the damage of renal tissue was much severer,the mRNA and protein expressions of GRP78 and Caspase-12 were significantly increased(p<0.01) as well.Contrast to the I/R group,the serum BUN,Cr level and the mRNA and protein expressions of GRP78 and Caspase-12 in I/R+Tau group dropped dramatically(p<0.05),the damage of renal tissue was lower.Hence,taurine could attenuate renal I/R injury,and might be one of the protective mechanism by down-regulating the over expressions of genes GRP78 and caspase-12.
2012, Vol. 36 
