Abstract：Objective To investigate the correlation between psychological stress and decrease in ovarian store.Methods Forty mice were exposed to an restraint stress protocol to induce psychological stress.These mice were randomly divided into four groups:control group and 3 stress groups treated with restraint for 1,2 and 4 weeks,respectively.At 24 hours after the end of restraint,blood samples were collected from the orbit.Serum cortisol levels were examined by radioimmunoassay and anti-Müllerian hormone(AMH) levels were measured by ELISA.Results Compared with controls,the cortisol levels were increased and AMH levels were decreased by restraint stress for 1 or 4 weeks(P<0.05),but levels of cortisol and AMH were not obviously changed by restraint stress for 2 weeks(P>0.05).Moreover,restraint stress for 1 week elevated cortisol levels but reduced AMH levels compared with restraint stress for 4 weeks(P<0.05).The relevant analysis indicated that cortisol levels were negatively correlated with AMH levels(r=-0.990,P<0.01).Conclusion Psychological stress can cause the decrease in ovarian store.
2012, Vol. 52 
