KONG Wei-guo a,WU Xiao-mu a,ZHANG Kun-nan a,HU Guo-zhu b,DING Wei-rong b,HE Dan b(a.Department of Neurology;b.Institute of Clinical Medicine,the People’s Hospital of Jiangxi Province,Nanchang 330006,China)
摘要目的探讨人骨髓间充质干细胞(human bone marrow mesenchymal stemcells,hMSCs)在体外诱导向运动神经元分化的潜能。方法用密度梯度离心法分离hMSCs,在含有10%FBS的DMEM/F12培养基中的培养扩增。将传代后的第1代hMSCs分为诱导组和对照组,诱导组的细胞在完全培养基中加入终浓度
Abstract：Objective To explore the differentiation potentiality of human bone marrow mesenchymal stem cells (hMSCs) into motor neurons in vitro.Methods The hMSCs were isolated from fresh bone marrow by a method of density gradient centrifugation,then amplified and
2009, Vol. 51 