褶纹冠蚌热休克蛋白HSP70基因的克隆及表达研究
谢彦海; 胡宝庆; 文春根
南昌大学生物科学系; 南昌大学实验动物科学部;
XIE Yan-haia,b,HU Bao-qinga,WEN Chun-gena(a.Department of Bioscience;b.Department of Laboratory Animal Science,Nanchang University,Nanchang 330031,China)
摘要 运用兼并引物结合RT-PCR方法从褶纹冠蚌(Cristaria plicata)血细胞中克隆出热休克蛋白70(HSP70)的cDNA序列。用半定量PCR方法研究了HSP70mRNA在褶纹冠蚌不同组织的表达情况。结果表明:HSP70cDNA全长为2 664bp,5’非编码区364个核苷酸,3’非编码区383个核苷酸,开放阅读框为1 917
关键词 :
嗜水气单胞菌 ,
褶纹冠蚌、热休克 ,
HSP70、表达
Abstract :The sequences of HSP70 cDNA were cloned from the haemocytes of freshwater mussel Cristaria plicata using degenerate primers to integrate the reverse transcription-polymerase chain reaction(RT-PCR).The Semi-quantitative PCR method was applied to further st
Key words :
heat shock
HSP70
expression
aeromonas hydrophila;
Cristaria plicata
出版日期: 2011-10-28
引用本文:
谢彦海; 胡宝庆; 文春根. 褶纹冠蚌热休克蛋白HSP70基因的克隆及表达研究[J]. 南昌大学学报(理科版), 2011, 35(05): 1-.
XIE Yan-haia,b,HU Bao-qinga,WEN Chun-gena(a.Department of Bioscience;b.Department of Laboratory Animal Science,Nanchang University,Nanchang 330031,China). . , 2011, 35(05): 1-.
链接本文:
http://qks.ncu.edu.cn/Jwk_xblxb/CN/ 或 http://qks.ncu.edu.cn/Jwk_xblxb/CN/Y2011/V35/I05/1