Abstract：Objective To study the pharmacokineties of rhizoma extract notopterol in rats.Methods Blood sampling was performed at different time points after intragastric administration of notopterol(15,30 and 60 mg·kg-1) to rats.Plasma notopterol concentrations were determined by high-performance liquid chromatography(HPLC) after protein precipitation with acetonitrile.The analytes were separated on a Spherisorb 10 ODS(1)column(250 mm×4.6 mm) with a mobile phase consisting of methanol-acetonitrile-0.025 mol·L-1 triethylamine phosphate(15∶45∶40).The flow rate was 1 mL·min-1 and the eluent was detected at 480 nm.The pharmacokinefic parameters were calculated by DAS 2.0.Results The linear range of notopterol in plasma was 1.164×10-4~1.164×10-2 mg·mL-1(r=0.997) and the recovery rate was more than 80%.Intra and inter-days precision and accuracy RSD were less than 4% and the obtained recovery rate was more than 90 %.Pharmacokinetic parameters were as follows:Cmax was(5.243±3.116),(1.892±0.888) and(0.764±0.120) mg·L-1,AUC(0-∞) was(32.658±31.202),(17.511±2.287) and(3.246±2.432) mg·L-1·h-1,and T1/2z was(3.184±1.959),(3.207±3.036) and(3.013±3.225) hours in 60,30 and 15 mg·kg-1notopterol treatment groups,respectively.Excellent linearity was obtained between notopterol doses and AUC.No significant differences were found in T1/2z among the three doses of notopterol(P>0.05).Conclusion The HPLC-fluorescence method is stable,sensitive and specific for determining the concentrations of notopterol in rat plasma.Pharmacokinetic trial showed that notopterol underwent absorption,rapid elimination of rapid metabolism in rats.These results can provide a reference for the next new drug trial research.
李云霞；郑晓鹤；孟宪生. HPLC测定大鼠体内羌活醇血药浓度及药代动力学研究[J]. 南昌大学学报（医学版）, 2012, 5(07): 7-.
Li Yun-xia1,ZHENG Xiao-he1,MENG Xian-sheng2. Determination of Notopterol Concentrations and Pharmacokinetics in Rat Plasma by High-performance Liquid Chromatography. , 2012, 5(07): 7-.
2012, Vol. 5 
