Abstract：Objective To explore a new biological therapy in fibrosis through inhibiting fibroblast collagen synthesis by soluble IL-13 receptor α 2(sIL-13Rα2).Methods The pMSCV/sIL-13 Rα2 vector was constructed and cell model expressing sIL-13 Rα2 was established.The medium was concentrated by ultrafiltration(0.2×106-molecular-weigh out off) and sIL-13 Rα2 was analyzed by Ni2 affinity chromatography,SDS-PAGE and Western blot.The expression of type Ⅰ collagen protein in fibroblasts was detected by RT-PCR and hydroxyproline method.Results HEK293 cells were successfully transfected with the constructed recombinant pMSCV/sIL-13 Rα2 and expressed sIL-13 Rα2.Type Ⅰ collagen synthesis was promoted by 50 μg·L-1 IL-13 in scar fibroblast CRL-1762 cells.But the promoting effect of IL-13 was inhibited by 50 μmol·L-1 sIL-13 Rα2.Conclusion Type Ⅰ collagen synthesis can be inhibited by targeting IL-13 with sIL-13 Rα2.in fibroblasts.
2012, Vol. 52 
