Abstract：Objective To establish an animal model of diet-induced obesity(DIO),and to investigate the efficacy and safety of weight-loss drugs in pre-clinical animal testing.Methods A total of 120 C57BL/6J mice were randomly divided into fed high-fat diet(Research Diets Inc.,D12492,60%kcal% fat;DIO group,n=110) or standard chow(control group,n=10) for 12 weeks.Food intake and body weight were measured weekly.The levels of plasma glucose,insulin and lipid were detected by enzyme-linked immunosorbent assay(ELISA).Blood samples were collected by tail cutting for glucose tolerance test.Results After feeding for 12 weeks,80.9% of mice in DIO group developed obesity and had impaired glucose tolerance,and hyperlipidemia.Compared with control group,body weight was significantly increased in DIO group [(36.90±5.07)g vs(27.68±2.27)g,P<0.001].Moreover,food intake and levels of plasma glucose and insulin obviously elevated compared with control group(P<0.01).Conclusion High-fat diet feeding can induce DIO and increase body weight in mice.The established model is closed with clinical obesity with the advantages of good stability,simple operation and low cost.
胡晓东； 李鸿炎. 饮食诱导小鼠肥胖疾病模型的建立[J]. 南昌大学学报（医学版）, 2012, 52(08): 6-.
HU Xiao-dong,LI Hong-yan. Establishment of A Mouse Model of Diet-induced Obesity. , 2012, 52(08): 6-.
2012, Vol. 52 
