Abstract：Objective To observe the changes in behaviors of hypoxic-ischemic encephalopathy(HIE)mice treated with neural stem cells(NSC),and to provide support for treating HIE with NSC,and to verify the feasibility of rotarod test,cylinder test and Morris water maze for measuring neurological deficit and recovery.Methods After HIE was induced,30 7-day-old mice were randomly assigned to receive NSC(NSC group,n=10),PBS(PBS group,n=10) and sham operation(SHAM group,n=10).Rotarod Test and Cylinder Test were performed at 7,14,21,28 and 35 days after treatment and Morris water maze test was carried out at 1-5 days,and 2 and 6 months after treatment to measure the behaviors of mice.Results 1) Rotarod Test : motor function recovered gradually and restored to near pre-operative levels on the 21th day in NSC group,and there was a significant difference between NSC group and PBS group on the 14th day(P< 0.05).2) Cylinder Test : motor function recovered with time in NSC group,and use frequency of ipsilateral forelimb was significantly different between NSC group and PBS group at 28days after treatment(P<0.05).3) Morris water maze test : escape latency shortened gradually with increasing training days,and the differences were significant among the three groups during the first 5 days after treatment(P<0.001).Compare with results at 2months after treatment,escape latency obviously decreased at 6months after treatment in NSC group(P<0.05).Compared to PBS group,escape latency significantly shortened in NSC group at each time point(P<0.05).Conclusion Rotarod Test is mainly applied to the detection of acute neurological disorder and recovery.Morris water maze test can be used to determine the changes in behaviors of HIE mice treated with NSC in the later period.Cylinder Test is a simple and economic experiment,which can be applied to neurobehavioral assessment in any period and may be used in conjunction with other behavioral test.
2012, Vol. 52 
