摘要用香草醛-高氯酸比色法测定苦瓜总皂苷。通过对比色条件进行优化得最佳条件:加热时间15 m in,加热温度60℃,显色剂用量0.7 mL 8%香草醛-冰乙酸。结果表明,该测定方法精密度高,在50 m in内显色稳定,加样回收率高,平均加样回收率为101.46%,RSD为1.19%(n=3),且操作简单,对设备要求
Abstract:The contents of total mormodicoside in Mormordica charantia L.were determined by Vanillin-HCLO4 colorimetry.The suitable conditions for colorimetry were obtained by optimization of determination with the heating time of 15 min,the heating temperature of 6