Abstract：The effect of different hormone sort and concentration on multiplication,differentiation and rooting of the rhizome of Cymbidium ensifolium was investigated,The variation of nutrient consumption during the rhizome multiplication was recorded as well.The results indicated that the best condition for rhizome multiplication was the MS+0.5 mg·L-1 6-BA+30 g·L-1 Sucrose,with the growth rate being 7.33;while the MS medium containing 0.6 mg·L-1 NAA,1.0 mg·L-1 TDZ and 30 g·L-1 Sucrose could be optimized for differentiation of rhizome,whose differential rate reached 93.10%.The 1/2 MS supplemented with 1.0 mg·L-1 NAA,30 g·L-1 Sucrose and 0.05 active carbon became the best rooting medium,in which rooting rate reached 96.3%.The growth change of rhizome proliferation was S-shaped curve.Boundaries were not clearly in various periods,which was different from the cell suspension culture system.The consumption of carbon,nitrogen and phosphorus were consistent with those in the rhizome growth curve.The pH of medium firstly decreased and then increased,for the sequence of consumption of ammonium and nitrate.
2012, Vol. 36 
