Abstract： The effects of the water-soluble ingredients of lithospermum-lithosperman,on progesterone secretion induced by hCG in rat luteal cells Utilizing the in vitro cultured luteal cells was studied,through the in vitro cultured luteal cells.The related mechanisms were investigated,too.The Luteal cells were isolated from immature female SD rats selected and divided into control and hCG group.Different concentrations of lithosperman were added into each group.Afterwards,the contents of both intercellular progesterone and the progesterone in culture solution were detected by radioimmunity.In order to reveal the onset time and kinetic features of lithosperman on hCG secretion,the cell suspension was divided into four groups:control group,1.50 g·L-1 lithosperman group,2×104 U/L hCG group and hCG(2×104 U/L)+lithosperman(1.50 g·L-1) group.The contents of progesterone were then examined at different cultured time points(0,0.5,1 or 2 h) in each group.In addition,the cells after 0.5 h culture were centrifuged and separated from the culture solutions.The total cAMP and cGMP were obtained by summing up the contents in cells and solutions,and the cAMP/cGMP ratios was calculated.The results showed:1.Lithosperman suppressed the hcG-induced progesterone production in luteal cells in a dose-dependent manner.2.Lithosperman in high dose(>3.00 g·L-1) could inhibit the basic progesterone generation in the controlled group.3.In the concentration of 1.50 g·L-1 group,lithosperman started to significantly inhibit the hCG-induced progesterone generation after 0.5 h incubation.4.hCG obviously increased the cAMP content in luteal cells,resulting in the elevation of cAMP/cGMP ratio.However,the net effect was that cAMP/cGMP ratio decreased although the addition of 1.50 g·L-1 lithosperman increased the contents of both cAMP and cGMP.Conclusions:Lithosperman could inhibit the hCG-induced hormone secretion in luteal cells,which are the targets of gonadotropins.Furthermore,the quick effect of lithosperman hints that it might be mediated by cGMP. 还原
2012, Vol. 36 
