Influence of 2(4-hydroxy-3-methoxycinnamon acyloxymethyl)-3,5,6-trimethylpyrazine on Expression of ICAM-1 in Endothelial Cells Injured by Hydrogen Peroxide
Abstract：Objective To investigate the protective effect of 2-(4-hydroxy-3-methoxycinnamon acyloxymethyl)-3,5,6-trimethylpyrazine(TMPF)on oxidative damage in human umbilical vein endothelial cells(ECV304),and to explore the mechanism of TMPF action.Methods The ECV304 cells were damaged by hydrogen peroxide and the protective effects of TMP and TMPF on oxidative damage were observed(Determination of MDA content and SOD vitality according to kit instructions;Determination of expression of ICAM-1 by real-time PCR;Determination of the expression of ICAM-1protein by Elisa).Results Compared with normal control cells,hydrogen peroxide induced an increased in malondialdehyde(MDA) content and a decrease in superoxide dismutase(SOD) activity(P<0.05or P<0.01).However,TMP or TMPF treatment sifnificantly inhibited hydrogen peroxide-induced MDA formation and increased SOD activity(P<0.05or P<0.01).Compared TMP treatment,TMPF treatment reduced MDA formation and elevated SOD activity.But no significant reduction in SOD activity was obtained by treatment with TMP or TMPF at dose of 12.5 μ mol.L-1(P>0.05).The expression of ICAM-1mRNA in normal control group,25.0 μ mol.L-1 TMP treatment group and 25.0 μ mol.L-1 TMPF treatment group was 0.245,0.454 and 0.376 times higher than that in hydrogen peroxide treatment group,respectively.Compared with hydrogen peroxide treatment group,the expression of ICAM-1protein significantly decreased in normal control group,25.0 μ mol.L-1 TMP treatment group and 25.0 μ mol.L-1 TMPF treatment group(P<0.05or P<0.01).Conclusion TMPF can protect hydrogen peroxide-induced oxidative damage,decrease MDA formation and increased SOD activity.TMPF may reduce ICAM1expression to attenuate oxidative damage in endothelial cells.
2012, Vol. 52 
