ZHANG Yi-daa,CAO Run-fub (a.2007 Grade of Medical Department of Graduate School;b.Department of Urinary Surgery,the First Affiliated Hospital,Nanchang University,Nanchang 330006,China)
摘要目的研究在γ射线诱导下PUMA基因(p53 up-regulated modulator of apoptosis)在人前列腺癌细胞(p53野生型LNCaP细胞、p53变异型PC-3细胞)中的作用效果。方法采用MTT方法检测在3、5、8Gy照射剂量的γ射线诱导下人前列腺癌细胞的抑制率;采用RT-PCR的方法检测在3、5、8Gy照射剂量
Abstract:Objective To study the effect of γ-rays on PUMA (p53 up-regulated modulator of apoptosis)gene in human prostate cancer cells.Methods The mortality of the human prostate cancer cells induced by 3,5,8 Gy dose of γ-rays was detected by MTT assay and the ex