Abstract:The interaction between simazine and calf thymus DNA in a pH 7.4 Tris-HCl buffer was investigated with the use of acridine orange(AO) dye as a fluorescent probe by fluorescence,UV-vis absorption,circular dichroism(CD),and Fourier transform infrared(FT-IR) spectroscopy,as well as DNA melting studies,viscosity measurements and salt effect.It can be concluded that simazine molecules could intercalate into the base pairs of DNA as evidenced by slgnificant fluoresce ce quenching of the DNA-AO complex with the binding of simazine to DNA by substituting for AO in the DNA-AO complex,and increase in melting temperature and relative viscosity of DNA.Furthermore,the FT-IR spectroscopy and salt effect demonstrated that there exists electrostatic attraction between simazine and DNA simultaneously.The thermodynamic parameters suggested that the binding of simazine to DNA was driven mainly by hydrophobic interactions.