ZHANG Kun-nan1,XU Zi-qiang2,WANG Chao-dong3,QU Xin-hui1,XIE Xu-fang1,WU Xiao-mu1(1.Department of Neurology,the People’s Hospital of Jiangxi Province,Nanchang 330006,China;2.2007 Grade of Medical Department of Graduate School,Nanchang University,Nanchang
摘要目的建立一种检测结核分枝杆菌特异性抗原培养滤液蛋白10(culture filtrate protein 10,CFP10)的斑点酶联免疫吸附试验(dot enzyme linked i mmunesorbant assay,Dot-ELISA)方法。方法采用真空泵抽吸浓缩检测样本,改进Dot-ELISA用改进前的和改进后的Dot-ELISA方法检测同一批临
Abstract:Objective To develop a dot enzyme linked immunosorbent assay(Dot-ELISA)method for detecting culture filtrate protein 10(CFP10).Methods Dot-ELISA method was improved using vacuum pump to concentrate test samples.Twenty-five cases of tuberculous pleural eff